Non-dehiscent sesame variety S25

ABSTRACT

A non-dehiscent sesame ( Sesamum indicum  L.) designated S25 has been found. Its degree of shatter resistance, or seed retention, makes S25 suitable for mechanized harvesting.

BACKGROUND

[0001] Sesame, or Sesamum indicum, is a tropical annual cultivated worldwide for its oil and its nut flavored seeds. In the Middle East, sesame is consumed as tahini, a sesame butter or sesame paste which is often mixed with ground chickpea kernels in a food preparation called hummus. Sesame is used widely in China, Japan, and Korea as a cooking oil, and it is consumed for its medicinal qualities. In recent years, the Japanese have been identifying and quantifying the medicinal benefits of sesame. In vitro studies and animal studies have indicated several antioxidant properties of sesame (Namiki, M. 1995. “The chemistry and physiological functions of sesame,” Food Rev Int 11:281-329). In the western hemisphere, sesame is primarily used in the confectionary trade in rolls and crackers. Throughout the world, sesame seeds or paste are mixed into sweets, e.g., halva. Sesame oil use in the cosmetic industry continues to expand.

[0002] The sesame plant grows to a height of about 56-249 cm, and at its leaf axils are found capsules which contain the sesame seed. Upon maturity in nature, the capsules holding the sesame seeds begin to dry down, the capsules normally split open, and the seeds fall out. Commercially, the harvester tries to recover as much seed as possible from mature capsules. From ancient times through the present, the opening of the capsule has been the major factor in attempting to successfully collect the seed. Harvesting methods, weather, and plant characteristics all contribute to the amount of seed recovered.

[0003] The majority of the world's sesame is harvested manually. With manual non-mechanized methods, it is desirable for the sesame seed to fall readily from the plant. Upon physiological maturity, the sesame stalks are cut, tied into small bundles, and then stacked in shocks. Further harvesting procedures vary from country to country and from area to area within countries. Some move the shocks to a threshing floor so that the seed that falls out can be recovered. Others put plastic or cloth in the fields under the shocks to catch the seed. For manual harvesting methods in which the dried, shocked sesame is moved to a threshing floor or over a plastic or cloth, preferred plant varieties include dehiscent, or super shattering, in which less than 10% of the seeds set are retained in the capsule.

[0004] Other methods involve leaving the shocks in the fields, and when the shocks are dry, the sesame is turned upside down and struck with an implement to shake out all of the seed. For this type of manual harvesting method, it is preferred that the capsule hold as much of the sesame seed as possible until the farmer inverts the stalk. Plant varieties rated as shattering which retain as much seed as possible before inversion are preferred. Common methods of manual harvest are discussed in Weiss, E. A. “Sesame”, Oilseed crops (2^(nd) ed.), Chapter 5, Blackwell Science, Inc., Malden, Mass., p.131-164 (2000).

[0005] In an effort to mechanize the harvest of sesame, D. G. Langham introduced the use of swathers in Venezuela in 1944. The swathers were used to cut the sesame plants, manual labor was used to bundle and shock the cut plants, and combines were brought in to thrash the shocks. It was determined that seed shattering during mechanized harvesting methods caused considerable loss of sesame seed. While mechanization was considered to be essential for crop production in the Western hemisphere, it became obvious that the dehiscence of the sesame seed pod was the principal obstacle to the widespread acceptance of sesame as a commercial crop. (Langham, D. G. 1949. “Improvement of Sesame in Venezuela,” Proceedings First International Sesame Conference, Clemson Agricultural College, Clemson, S.C., pp. 74-79). As programs to introduce sesame production in the United States in Arizona, South Carolina, Nebraska, Oklahoma, and Texas were initiated, mechanization was considered essential due to high labor costs. Kalton, one of the Texas researchers, reported that the shattering nature of available strains was the main obstacle in complete mechanization of the sesame crop. (Kalton, R. 1949. “Sesame, a promising new oilseed crop for Texas,” Proc First International Sesame Conference, Clemson Agricultural College, Clemson, S.C., pp. 62-66).

[0006] In 1943, D. G. Langham found a mutation on a sesame plant. Capsules did not open on plants expressing this mutation. In succeeding generations, Langham showed that it was a recessive single gene which produced this indehiscence, where all the seeds were retained inside the unopened capsule. While it was believed that indehiscence would solve the problem of seed loss on mechanized harvesting, it was found that the capsules were too tough to effectively release the seed. Many of the capsules passed through a combine without opening. When more rigorous combining was attempted, an increase in efficiency of capsule opening was achieved but at the expense of seed quality. Seeds were broken due to the more rigorous combine conditions, and the broken seeds released free fatty acids. Chemical reactions with free fatty acids led to rancidity and concomitant undesirability of the harvested seed.

[0007] The indehiscent sesame varieties described above were used by various plant breeders in an attempt to develop desirable sesame lines. In addition to traditional cross-breeding approaches, some attempted to alter the chromosome numbers through tetraploids and interspecific crosses. Yermanos attempted to improve release of seed by increasing the length of the capsule so that there would be more surface for the combine to crack the capsules open (personal communication). Unfortunately, even with a small opening on the top of the capsule, a high percentage of broken seed was found on mechanized harvesting, preventing commercial use of this sesame line.

[0008] D. G. Langham reported in the late 1950's that the placenta attachment between each sesame seed and the placenta was important in the retention of seed in the capsule. He believed that he could improve the shatter resistance of sesame with increased placenta attachment but did not believe that all the seed could be retained in the capsule (Langham, D. G., Rodriguez, Maximo, and Reveron Esteban. 1956. “Dehiscencia y otras caracteristicas del ajonjolí, Sesamum indicum L., en relación con el problema de la cosecha”, Genesa, Maracay, Venezuela, pp. 3-16). However, Yermanos reported that during capsule maturity, the placenta attachment gradually weakens and is obliterated when the capsule is completely desiccated. (Yermanos, D. M. 1980. “Sesame. Hybridization of crop plants,” Am Soc Agronomy-Crop Sci of America, pp. 549-563). Thus, it appeared that the placenta attachment would have little effect on seed retention in dry, mature capsules during harvesting. A seamless gene which retained all the seed in the capsules was discovered by D. G. Langham and D. R. Langham in 1986. This was crossed with shattering types, and some progeny had an opening at the tip of the capsule. The seamless capsules were similar to the indehiscent capsules in that it was too difficult to remove the seed from the capsule without damaging the seed.

[0009] In 1982, the first non-shattering line (retaining 50-70% of the seeds set) requiring no manual labor was introduced. This line could be harvested by swathing the sesame, leaving it to dry in the field, and then picking it up by a combine. Although complete mechanization was achieved, extensive loss of seed due to adverse weather conditions continued to occur. (Langham, D. R., “Shatter resistance in sesame”, In: L. Van Zanten (ed.), Sesame improvements by induced mutations, Proc. Final FAO/IAEA Co-ord. Res. Mtng, IAEA, Vienna, TECDOC-1195, p.51-61 (2001)).

[0010] Other varieties were developed between 1988 and 1997 which allowed for direct combining with 70-90% seed retention, but extensive loss of seed due to wind and rain continued to occur. Lines that generally yielded 80% of the seed under ideal conditions would yield only 45-65% under adverse conditions. Thus, while many of the crosses began to moderate the deleterious effects of mechanized harvesting, none were able to increase the yields to the level of manually harvesting shattering cultivars.

[0011] U.S. Pat. No. 6,100,452 which issued Aug. 8, 2000, disclosed non-dehiscent sesame lines Sesaco 22 (S22), Sesaco 23 (S23), Sesaco 24 (S24), 19A, and 11W, representative seed having been deposited under ATCC accession number PTA-1400, PTA-1401, PTA-1402, PTA-1399, and PTA-1398, respectively. These sesame lines are characterized by their high degree of seed retention within the capsule despite adverse weather conditions such as wind and rain and the retention of a sufficient amount of sesame seed during mechanized harvesting to be competitive with manual harvesting with minimization of seed breakage.

[0012] A non-dehiscent variety designated S25 has now been found which provides some improvements over previously disclosed non-dehiscent sesame lines.

SUMMARY OF THE INVENTION

[0013] In one aspect, the present invention is seed of a sesame plant S25, a sample of the seed having been deposited under ATCC Accession No. ______.

[0014] In another aspect, the present invention is a sesame plant or its parts produced by growing the seed of sesame plant S25, a sample of the seed having been deposited under ATCC Accession No. ______. Pollen is one of the sesame plant parts of the present invention.

[0015] In another aspect, the present invention is a sesame plant having all the physiological and morphological characteristics of sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.

[0016] In another aspect, the present invention is a sesame plant having all the physiological and morphological characteristics of a sesame plant produced by growing the seed having been deposited under ATCC Accession No. ______.

[0017] In another aspect, the present invention is a sesame plant or its parts having as a parent sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.

[0018] In another aspect, the present invention is seed from a progeny sesame plant having as a parent sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.

[0019] In another aspect, the present invention is seed from a progeny sesame plant having as a parent a sesame plant produced by the seed having been deposited under ATCC Accession No. ______.

[0020] In another aspect, the present invention is a tissue culture of seed having been deposited under ATCC Accession No. ______.

[0021] In another aspect, the present invention is a tissue culture of sesame plant S25 or its parts, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.

[0022] In another aspect, the present invention is a sesame plant regenerated from a tissue culture of a seed having been deposited under ATCC Accession No. ______, wherein the regenerated sesame plant has all the physiological and morphological characteristics of a sesame plant produced by the seed deposited under ATCC Accession No. ______.

[0023] In another aspect, the present invention is a sesame plant regenerated from a tissue culture of sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______, wherein the regenerated sesame plant has all the physiological and morphological characteristics of sesame plant S25.

[0024] In another aspect, the present invention is a method of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, wherein the first or second parent sesame plant was produced by seed having been deposited under ATCC Accession No. ______.

BRIEF DESCRIPTION OF DRAWINGS

[0025]FIG. 1 depicts the lineage of S25.

[0026]FIG. 2 depicts a comparison of the percent of seed retention during in shaker shatter resistance testing from 1997 to 2001 for sesame varieties released by Sesaco: Sesaco 01 (S01) released in 1982, Sesaco 02 (S02) released in 1983-1987, Sesaco 03 (S03) released in 1985-1986, Sesaco 04 (S04) released in 1984-1987, Sesaco 05 (S05) released in 1984, Sesaco 06 (S06) released in 1984, Sesaco 07 (S07) released in 1985-1988, Sesaco 08 (S08) released in 1986, Sesaco 09 (S09) released in 1985-1986, Sesaco 10 (S10) released in 1988-1991, Sesaco 11 (S11) released in 1988-1995, Sesaco 12(S12) released in 1986-1987, Sesaco 14 (S14) released in 1989, Sesaco 15 (S15) released in 1990-1991, Sesaco 16 (S16) released in 1991-1996, Sesaco 17 (S17) released in 1993-1998, Sesaco 18 (S18) released in 1994-1996, Sesaco 19 (S19) released in 1994-1995, Sesaco 20 (S20) released in 1995-1997, Sesaco 21(S21) released in 1995-1998, Sesaco 22 (S22) released in 1997-1998, Sesaco 23 (S23) released in 1998-2000, Sesaco 24 (S24) released in 1998-2002, and Sesaco 25 (S25) released in 2001-2002.

[0027]FIG. 3 depicts a comparison of the kill resistance ratings in Uvalde, Tex., in 2001 for the varieties released by Sesaco.

[0028]FIG. 4 depicts a comparison of the mean days to physiological maturity from 1998 to 2001 for the varieties released by Sesaco.

[0029]FIG. 5 depicts a comparison of the mean weight of 100 seeds in grams from 1997 to 2001 for the varieties released by Sesaco.

DETAILED DESCRIPTION

[0030] Sesame cultivar S25 is a non-dehiscent sesame variety having superior characteristics which make it a commercially suitable sesame line. S25 exhibits non-dehiscence as defined in U.S. Pat. No. 6,100,452, the teachings of which are incorporated herein by reference, making it suitable for mechanized harvesting. In addition, S25 has an early maturing cycle which extends the planting region to more northern latitudes and improved resistance against common fungal diseases.

[0031] The recommended geographical area for S25 is from South Texas at the R10 Grande to southern Kansas and from east Texas westward to elevations below 1,200 meters. S25 has not been tested in other states, but it is projected that it would perform well in N. Mex., Arizona, and California. S25 has not been exposed to the sesame diseases that have been reported east of Texas.

[0032] Sesaco used the pedigree method of plant breeding to develop S25. Sesame is generally self-pollinated. Crossing is done using standard techniques as delineated in Yermanos, D. M. 1980. “Sesame. Hybridization of crop plants,” Am Soc Agronomy-Crop Sci of America, pp. 549-563 and U.S. Pat. No. 6,100,452. Ashri provides an overview of sesame breeding in Ashri, A. (1998). “Sesame breeding,” Plant Breed. Rev. 16:179-228.

[0033] The lineage of S25 is presented in FIG. 1. 192 (1) was a line obtained from the M. L. Kinman in 1980 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. The line was originally T61429-B-4-1-3 from the Kinman United States Department of Agriculture (USDA) sesame program, College Station, Tex. which had been in cold storage at Ft. Collins, Colo. In 1997, the line was transferred to the National Plant Germplasm System (NPGS), Griffin, Ga. and given the identifier P1599462. Within Sesaco, 192 first carried the identifier 1479 and then was changed to X191 and X193. In 1985, a selection from X193 became Sesaco 3 (S03) and a selection of X191 became Sesaco 7 (S07).

[0034] 888 (2) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, 888 first carried the identifier F888 and was later changed to T888.

[0035] V52 (3) was a cultivar designated as SF075 obtained from the Sesamum Foundation (D. G. Langham, Fallbrook, Calif.) collection in 1977 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. The Sesamum Foundation obtained it from B. Mazzani (Maracay, Venezuela) in 1960. Originally, it was a cultivar known as Venezuela 52 developed by D. G. Langham in the 1940s. Within Sesaco, V52 first carried the identifier 0075 and was later changed to TV52.

[0036] K0367 (4) was a cross made by Sesaco in the Hancock nursery (Wellton, Ariz.) in 1986. Within Sesaco, it has carried the identifier K0367.

[0037] G8 (5) was a line obtained from D. G. Langham in 1977 and first planted by Sesaco in the Kamman nursery (Wellton, Ariz.) in 1978. It was a selection from the cultivar ‘Guacara’ which D. G. Langham developed in Venezuela in the 1950s. Guacara was an initial selection from a cross that later produced one of the major varieties in Venezuela—Aceitera. Within Sesaco, G8 first carried the identifier XO 11 and was later changed to TG8.

[0038] 804 (6) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it has carried the identifier F804; in 1988, a selection of this line became Sesaco 11 (S11).

[0039] 111 (7) was a line obtained from the NPGS (PI173955) in 1979 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. NPGS obtained it in 1949 from W. N. Koelz, USDA, Beltsville, Md. who obtained it from India. Within Sesaco, 111 first carried the identifier 0858 and was then changed to X111. In 1985, a selection of this line became Sesaco 4 (S04).

[0040] 111X (8) was an outcross in the plot BT0458 in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it carried the identifier E0745.

[0041] 88B (9) was a cross made by Sesaco in the Wright nursery (Tacna, Ariz.) in 1987. Within Sesaco, it first carried the identifier KAC32 and was later changed to X88B and then to T88B.

[0042] 56B (10) was a cross made by Sesaco in the Wright nursery (Tacna, Ariz.) in 1987. Within Sesaco, it first carried the identifier KAN00 and was later changed to X56B and then to T56B.

[0043] F822 (11) was a cross made by Sesaco in the Nickerson nursery (Yuma, Ariz.) in 1982. Within Sesaco, it has carried the identifier F822.

[0044] 562 (12) was a cross made by Sesaco in the McElhaney nursery (Wellton, Ariz.) in 1983. Within Sesaco, it first carried the identifier G8562 and was later changed to T562.

[0045] 700 (13) was a line obtained from the NPGS (PI292144) in 1979 and first planted by Sesaco in the Woods nursery (Wellton, Ariz.) in 1981. NPGS obtained it in 1963 from Hybritech Seed International, a unit of Monsanto, U.S., which obtained it from Israel. In viewing this material in 1986, A. Ashri of Israel concluded that it was an introduction to Israel. The material is similar to introductions from India and Pakistan. Within Sesaco, 700 first carried the identifier 0700 and was later changed to T700.

[0046] S25 (14) was a cross made by Sesaco in the Sharp nursery (Tacna, Ariz.) in 1989. Within Sesaco, it first carried the identifier N2248. The seed (C954) from the cross was planted in Plot W7163 in the Gilleland nursery (Uvalde, Tex.) in 1990, and five plants were selected based on the observation of “super hold/plants.” The seed (5870) of one of the plants was planted in Plot J673 in the Corley nursery (Lamesa, Tex.) in 1991, and two plants were selected based on the observation of “good plants.” The seed (5045) of one of the plants was planted in Plot B864 in the Gilleland nursery in 1992, and one plant was selected based on the observation of “very early.” The seed (8397) was planted in Plot 6041 in the Gilleland nursery in 1993, and three plants were selected based on the observation of “longer capsule zone.” The seed (8079) of one of the plants was planted in Plot VB14 in the Corley nursery in 1994, the identifier was changed to X6BF, and a bulk of 5 plants was selected based on the observation of “lot of capsules, placenta attachment in open capsules and good lodging resistance.” The bulk seed (7853) was planted in Plot 1684 in the Gilleland nursery in 1995, and a bulk of 19 plants was selected based on the observation of “super placenta attachment, quick drydown and best plot.” The bulk seed was planted in Plot 0811 in the Gilleland nursery in 1996, and a bulk of 54 plants was selected based on the observation of “best individuals, best visual placenta attachment, lower height, light seeking and asymmetrical branching.” The bulk seed (0006) was planted in Plot C161 in the Krehbiel nursery (Hydro, Okla.) in 1997, the identifier was changed to X24W, and a bulk of 7 plants was selected based on the observation of “lower height and wider row.” The bulk seed (1466) was planted in Plot F077 in the Meeks nursery (Tipton, Okla.) in 1998, and a bulk of 61 plants was selected based on the observation of “seed to the top, lots of capsules, good branches that are not broken, low plants, and went to drydown faster than other lines.” The bulk seed (1702) was planted in strip KS10 next to the Schwartz nursery (Wall, Tex.) in 1999, and the strip was combined. The combined seed was tested on the Bishop farm (Knippa, Tex.) and the Michalewicz farm (Water Valley, Tex.) in 2000, and the seed was combined. The combined seed was then released to farmers in May, 2001.

[0047] Along with breeding programs, tissue culture of sesame is currently being practiced in Korea, Japan, Sri Lanka and the United States. It is possible for one of ordinary skill in the art to utilize sesame plants grown from tissue culture as parental lines in the production of non-dehiscent sesame. Further, it is possible to propagate non-dehiscent sesame through tissue culture methods. By means well known in the art, sesame plants can be regenerated from tissue culture having all the physiological and morphological characteristics of the source plant.

[0048] The present invention includes the seed deposited under ATCC Accession No. ______; a plant, designated as S25, or parts thereof which are produced by the seed deposited under ATCC Accession No. ______; seed produced by plant S25 or its progeny having the physiological and morphological characteristics of sesame plant S25 or a plant produced by the seed deposited under ATCC Accession No. ______; any plant having plant S25 as a parent, and any plant having the physiological and morphological characteristics of sesame plant S25 or a plant produced by the seed deposited under ATCC Accession No. ______. The present invention also includes a tissue culture of seed having been deposited under ATCC Accession No. ______ or a tissue culture of sesame plant S25 or its parts, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______. A sesame plant regenerated from a tissue culture of a seed having been deposited under ATCC Accession No. ______ or from sesame plant S25, wherein the regenerated sesame plant has all the physiological and morphological characteristics of a sesame plant produced by the seed deposited under ATCC Accession No. ______ is also contemplated by the present invention. Methods of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, wherein the first or second parent sesame plant was produced by seed having been deposited under ATCC Accession No. ______ are part of the present invention.

[0049] Unless otherwise stated, as used herein, the term plant includes plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like. Further, unless otherwise stated, as used herein, the term progeny includes plants derived from plant cells, plant protoplasts, plant cell tissue cultures from which sesame plants can be regenerated, plant calli, plant clumps, plant cells that are intact in plants, or parts of plants, such as embryos, pollen, ovules, flowers, capsules, stems, leaves, seeds, roots, root tips, and the like.

[0050] Sesame cultivar S25 has shown uniformity and stability within the limits of environmental influence for the characters listed in Table I below. Table I provides the name, definition, and rating scale of each character as well as the method by which the character is measured. Under the rating section, the rating for S25 is presented in bold text. Additionally, the distribution of the character in Sesaco's sesame development program is indicated under the rating section. Sesaco uses slightly different character specifications from “Descriptors for sesame”, AGP:IBPGR/80/71, IBPGR Secretariat, Rome, (1981) and from the form “Sesame (Sesamum indicum)”, U.S. Department of Agriculture Plant Variety Protection Office, Beltsville, Md. The descriptors in those documents were developed in the early 1980s and have not been updated to incorporate new concepts in sesame data collection. TABLE I Characters Distinguishing the S25 Line Character Rating Methodology (1) BRANCHING Subjective rating The amount of branching on any particular STYLE Values: plant depends on the space around the plant. In The potential amount of U = Uniculm-no branching high populations, branching can be suppressed. true branching in a line except weak branches in This rating should be based on potential as open expressed on end plants and plants in the open. B = True branches True branches start in the leaf axil below S25 = B for all crops in all the first flower, and they begin to emerge nurseries before the first open flower. As long as there is Distribution within Sesaco light into the leaf axils, there will be additional based on stable lines in the branches that start below the first branches in crossing program in 1982- subsequently lower nodes. Weak branches 2001 (Total number of occur when a plant is in the open. They samples tested = 1,333) develop in the lowest nodes and subsequent U = 42.4% branches start at higher nodes. There are lines B = 57.6% that will not branch in any circumstance. Some lines in the open will put on spontaneous branches late in the cycle. True and weak branches do not have a capsule in the same leaf axil, whereas the spontaneous branches form under the capsule after the capsule has formed. Spontaneous branches are not counted as branches. There are rare lines where the flowering pattern is to put on flowers on lower nodes late in the cycle. In this case, the capsule is formed after the branch is developed. This pattern should not be termed spontaneous branching, and the branch is normally counted as a true branch. There are branched lines that have secondary branches on the branches. In a few cases, there can be tertiary branches. Additional branches generally appear in low populations. Comments: the effects of light appear to have more of an effect on branching than moisture and fertility. High populations suppress branching. (2) NUMBER OF Subjective rating Rating can be taken from about 60 days CAPSULES PER Values: after planting through to the end of the crop. LEAF AXIL 1 = Single Number of Capsules per Leaf Axil is highly The predominant number 3 = Triple dependent on moisture, fertility, and light. In of capsules per leaf axil in Based on potential as triple capsule lines, the central capsule forms the middle half of the described in the methodol- first, and axillary capsules follow a few days capsule zone ogy presented herein later. Triple capsule lines have the potential to S25 = 1 for all crops in all put on axillaries, but will not do so if plants do nurseries not have adequate moisture and/or fertility. In Distribution within Sesaco drought conditions, some triple capsule lines based on stable lines in the will produce only a central capsule for many crossing program in 1982- nodes. In these lines, when there is adequate 2001 (Total number of moisture through rain or irrigation, some will samples tested = 1,327) add axillary capsules on only new nodes, while 1 = 58.3% others will add axillary capsules to all nodes. 3 = 41.7% Some triple capsule lines will not put on axillary capsules if there is no direct sunlight on the leaf axil. To date, lines with single capsules have nectaries next to the central capsule in the middle of the capsule zone while triple capsules do not. However, some lines have what appear to be nectaries on the lower capsules of triple lines, but upon close examination, they are buds which may or may not eventually develop into a flower and then a capsule. In most triple capsule lines, the lower and upper nodes have single capsules. There are some lines where the end plants can put on 5 capsules/leaf axil and a few that have the potential to put on 7 capsules/leaf axil. 5 and 7 capsules only appear with open plants with high moisture and fertility. In some environments, single capsule lines will put on multiple capsules on 1 node and rarely on up to 5 nodes. These lines are not considered triple capsule lines. (3) MATURITY CLASS Subjective rating The basis for this data point is Days to The maturity of a line in Values: Physiological Maturity (Character No. 27). S24 relation to a standard line. V = Very early (<85 days) is the standard line to be used to compute Currently, the standard E = Early (85-94 days) Maturity Class. In 1998-2001, the maturity of line is S24 at 95 days M = Medium (95-104 days) S24 averaged 95 days in the Uvalde, TX, L = Late (105-114 days) nursery. For each line, the physiological T = Very late (>114 days) maturity for each year is subtracted by the S24 S25 = E in 1998-2001 in maturity for that year in that nursery, and then Uvalde nursery^(a) the number of days of difference is averaged. Distribution within Sesaco The average is then added to 95. based on stable lines in the See Days to Physiological Maturity crossing program in 1998- (Character No. 27) for the effects of the 2001 (Total number of environment on Maturity Class. samples tested = 650) V = 1.2% E = 26.8% M = 56.2% L = 12.9% T = 2.9% (4) PLANT Subjective rating The first character is the Branching Style PHENOTYPE Values: (Character No. 1), followed by the Number of A three character Branching Style Capsules per Leaf Axil (Character No. 2), and designation that provides U = Uniculm-no branching then the Maturity Class (Character No. 3). the branching style, except weak branches in When these characters are placed in a number of capsules per open matrix, there are 20 potential phenotypes. The leaf axil, and the maturity B = True branches phenotype provides an overview of the general class Number of Capsules per Leaf appearance of the plant. There is a very high Axil correlation between Maturity Class and Height 1 = Single of Plant (Character No. 5). 3 = Triple Maturity Class V = Very early (<85 days) E = Early (85-94 days) M = Medium (95-104 days) L = Late (105-114 days) T = Very late (>114 days) S25 = B1E for all crops in all nurseries Distribution within Sesaco based on stable lines in the crossing program in 1998- 2001 (Total number of samples tested = 650) U1V = 0% U3V = 1.1% U1E = 3.8% U3E = 8.3% U1M = 16.0% U3M = 12.0% U1L = 3.4% U3L = 2.2% U1T = 0.5% U3T = 0.6% BIV = 0% B3V = 0.2% B1E = 8.0% B3E = 6.3% B1M = 23.2% B3M = 4.8% B1L = 6.5% B3L = 1.0% B1T = 1.6% B3T = 0.4% (5) HEIGHT OF Unit of measure: cm The measurement is made after the plants PLANT Values: average of 3 plants stop flowering. For plants that are not erect or The height of the plant S25 = 125 cm in 2000 in have lodged, the plant should be picked up for from the ground to the top Uvalde nursery = the measurement. In most lines the highest of the highest capsule 126 cm in 2001 in capsule is on the main stem. In lines with the with viable seed Uvalde nursery dt/dt alleles (determinate), the highest capsule Distribution within Sesaco is on the branches. based on stable lines in the Comments: this height is dependent on the crossing program in 1999- amount of moisture, heat, fertility, and 2001 (Total number of population. Increased values generally increase samples tested = 2274) the height. In a high population, the height will low = 56 cm only increase if there is adequate fertility and high = 249 cm moisture; otherwise, the height will be shorter. 1 = <94.6 cm; 5.2% In low light intensities, the heights are 2 = <133.2 cm; 34.6% generally taller. 3 = <171.8 cm; 54.9% 4 = <210.4 cm; 5.1% 5 = >210.3 cm; 0.1% (6) HEIGHT OF FIRST Unit of measure: cm The measurement is made after the plants CAPSULE Values: average of 3 plants stop flowering. For plants that are not erect or The height of the first S25 = 49 cm in 2000 in have lodged, the plant should be picked up for capsule from the ground Uvalde nursery = the measurement. In most lines, the lowest to the bottom of the 41 cm in 2001 in capsule is on the main stem. True branches lowest capsule on the Uvalde nursery have capsules higher than on the main stem central stem Distribution within Sesaco except when the flowers fall off. Occasionally, based on stable lines in the on weak branches, the lowest capsule is on the crossing program in 1999- branches. 2001 (Total number of There are lines that flower in the lower samples tested = 2274) nodes late in the cycle, and, thus, the measure- low = 20 cm ment should be taken after flowering ends. In high = 193 cm many lines the first flower does not make a 1 = <54.6 cm; 52.7% capsule, and, thus, this height may not be the 2 = <89.2 cm; 45.5% same as the height of the first flower. The 3 = <123.8 cm; 1.5% height is correlated to the length of time to 4 = <158.4 cm; 0.3% flowering, the earlier the lower the height. 5 = >158.3 cm; 0.1% Comments: see Height of Plant (Character No. 5) for effects of environmental factors (7) CAPSULE ZONE Unit of measure: cm The measurement is derived by subtracting LENGTH Values: average of 3 plants the Height of First Capsule (Character No. 6) The length of the capsule S25 = 76 cm in 2000 in from the Height of Plant (Character No. 5). zone. The capsule zone Uvalde nursery = Comments: see Height of Plant (Character extends from the bottom 86 cm in 2001 in No. 5) for effects of environmental factors of the lowest capsule on Uvalde nursery the main stem to the top Distribution within Sesaco of the highest capsule on based on stable lines in the the main stem. crossing program in 1999- 2001 (Total number of samples tested = 2274) low = 18 cm high = 188 cm 1 = <52 cm; 4.7% 2 = <86 cm; 53.5% 3 = <120 cm; 41.3% 4 = <154 cm; 0.5% 5 = >153.9 cm; 0.1% (8) NUMBER OF Unit of measure: number The count is made after the plants stop CAPSULE NODES Values: average of 3 plants flowering. On alternate arranged leaves, each The number of capsule S25 = 26 in 2000 in Uvalde pair of leaves is counted as one node. In some nodes from the lowest nursery = lines, there are three leaves per node for at least capsule node to the 29 in 2001 in Uvalde part of the plant. In some plants, flowers may highest node with nursery not have produced capsules on one or more of capsules with viable seed Distribution within Sesaco the leaf axils in a node. These nodes should on the main stem of the based on stable lines in the still be counted. Nodes on the branches are not plant crossing program in 1999- counted. 2001 (Total number of In years when the amount of moisture samples tested = 2154) available to the plant is irregular, nodes can low = 10 become very irregular, particularly on triple high = 54 capsule lines. In the upper portions of the plant, 1 = <18.8; 17.9% it may become easier to count the capsule 2 = <27.6; 48.3% clusters and divide by 2. While it is possible to 3 = <36.4; 29.5% count nodes after leaves have fallen, it is much 4 = <45.2; 3.6% easier to count while the leaves are still on the 5 = >45.1; 0.7% plant. Comments: the number of nodes is dependent on the amount of moisture and fertility. Higher moisture and fertility increases the number of nodes. (9) AVERAGE Unit of measure: cm Divide the Capsule Zone Length (Character INTERNODE Values: average of 3 plants No. 7) by the Number of Capsule Nodes LENGTH WITHIN S25 = 3.0 cm in 2000 in (Character No. 8). CAPSULE ZONE Uvalde nursery = Comments: this length is dependent on the The average internode 3.0 cm in 2001 in amount of moisture, fertility, and population. length within the capsule Uvalde nursery Increased values generally increase the length. zone Distribution within Sesaco In a high population, the length will only based on stable lines in the increase if there is adequate fertility and crossing program in 1999- moisture; otherwise the length will be shorter. 2001 (Total number of In low light intensities, the lengths are samples tested = 2145) generally longer. low = 1.09 cm Past methodologies have measured the high = 8.09 cm internode length at the middle of the capsule 1 = <2.49 cm; 6.2% zone. Some have measured it at the median 2 = <3.89 cm; 74.6% node and others at the median Capsule Zone 3 = <5.29 cm; 18.6% Length. 4 = <6.69 cm; 0.4% 5 = >6.68 cm; 0.1% (10) YIELD AT Unit of measure: kg/ha On 3 replicated plots, when the plants are DRYDOWN Values: average of 3 dry enough for direct harvest, cut a minimum An extrapolation of the replications of 1/5000 of a hectare (Sesaco uses 1/2620) in yield of a field by taking S25 = 1343 kg/ha in 2000 in the plot and place the plants in a cloth bag. sample yields Uvalde nursery = Thresh the sample in a plot thresher and weigh 1614 kg/ha in 2001 in the seed. Multiply the weight by the Uvalde nursery = appropriate multiplier based on area taken to 87 kg/ha in 2000 in provide the extrapolated yield in kg/ha. San Angelo nursery^(b) In the Almaco thresher there is about 3% Distribution within Sesaco trash left in the seed. Since yields are compara- based on stable lines in the tive, there is no cleaning of the seed done crossing program in 1999- before the computation. If other threshers have 2001 (Total number of more trash, the seed should be cleaned before samples tested = 1828) weighing. low = 67 kg/ha Comments: yields increase with moisture high = 2421 kg/ha and fertility. However, too high a moisture can 1 = <537.8 kg/ha; 5.6% lead to killing of plants. Too high fertility can 2 = <1008.6 kg/ha; 15.6% lead to extra vegetative growth that may not 3 = <1479.4 kg/ha; 51.5% lead to higher yields. The optimum population 4 = <1950.2 kg/ha; 25.8% depends on the phenotype (Branching Style, 5 = >1950.1 kg/ha; 1.4% Character No. 1; Number of Capsules per Leaf Axil, Character No. 2; and Maturity Class, Character No. 3) and row width. (11) RESISTANCE TO Subjective rating In a year when there is a drought, this rating DROUGHT Values: 0 to 8 scale can be used to differentiate the effects of the The relative amount of 7 = Little effect from drought different lines. This is a highly subjective resistance to drought 4 = Medium effect from rating requiring a rater that is familiar with the drought performance of the line under normal 1 = Considerable effect from conditions. The rating is based on how the drought drought changes the line from normal. Thus, a Intermediate values are used. short line that does not change significantly in S25 = 2.5 in 2000 in San a drought may have a higher rating than a tall Angelo nursery line which is affected by the drought even Distribution within Sesaco though the taller line is taller in the drought based on stable lines in the than the short line. crossing program in 2001 (Total number of samples tested = 632) low = 0 high = 8 1 = <1.6; 0.8% 2 = <3.2; 28.0% 3 = <4.8; 36.1% 4 = <6.4; 34.5% 5 = >6.3; 0.6% (12) LEAF LENGTH Unit of measure: cm Select one leaf per node to measure from The length of the leaf Values: average of 3 plants the 5^(th), 10^(th), and 15^(th) nodes from the base of blade from the base of the S25 = 22.6 cm for 5^(th) node the plant. All the leaves for one line should be petiole to the apex of the in 2001 in San Angelo collected at the same time. Some lines retain leaf from the 5^(th), 10^(th), and nursery = the cotyledons, and the cotyledon node does 15^(th) nodes 18.7 cm for 10^(th) node not count as a node. In some lines the lowest in 2001 in San Angelo leaves abscise leaving a scar on the stem. nursery = Abscised nodes should be counted. In lines 14.8 cm for 15^(th) node with alternate leaves, one node is counted for in 2001 in San Angelo each pair of leaves. In some lines in parts of the nursery plant there are three leaves per node which Distribution within Sesaco should be counted as one node. for 5^(th) leaf based on stable The leaves continue growing in the first few lines in the crossing program days after they have separated from the in 2001 (Total number of growing tip. The choosing of leaves should be samples tested = 100) done a minimum of 5 days after the 15^(th) node low = 15.9 cm has appeared. Timing is important, because the high = 55.5 cm plants will begin to shed their lower leaves 1 = <23.8 cm; 41% towards the end of their cycle. 2 = <31.7 cm; 44% There are lines that have less than 15 nodes. 3 = <39.7 cm; 12% In this case, the highest node should be taken 4 = <47.6 cm; 1% and the node number annotated to the 5 = >47.5 cm; 2% measurements. Distribution within Sesaco There can be as much as 6 mm difference for 10^(th) leaf based on stable between a green leaf and a dry leaf. The lines in the crossing program measurements can be done on a green or dry in 2001 (Total number of leaf as long as any comparison data with other samples tested = 100) lines is based on the same method. low = 12.0 cm Generally, the lowest leaves increase in size high = 53.5 cm until the 4^(th) to 6^(th) node and then they decrease 1 = <20.3 cm; 48% in size. This applies to all measurements (Leaf 2 = <28.6 cm; 40% Length (Character No. 12), Leaf Blade Length 3 = <36.9 cm; 8% Character No. 13), Leaf Blade Width 4 = <45.2 cm; 3% (Character No. 14), and Petiole Length 5 = >45.1 cm; 1% (Character No. 15). Generally, the width will Distribution within Sesaco decrease at a greater rate than the length. for 15^(th) leaf based on stable Comments: the length is dependent on the lines in the crossing program amount of moisture and fertility. Higher in 2001 (Total number of moisture and fertility increase the length. Leaf samples tested = 59) size also appears to be affected by light low = 10.0 cm intensity. In Korea, the Korean lines have high = 49.5 cm much larger leaves than in Oklahoma. In 1 = <17.9 cm; 54.2% Korea, there is more cloud cover and a general 2 = <25.8 cm; 37.3% haze than in Oklahoma. 3 = <33.7 cm; 6.8% The largest leaves are on photosensitive 4 = <41.6 cm; 0% lines which when planted in the tropics fall into 5 = >41.5 cm; 1.7% the 2^(nd) and 3^(rd) class (13) LEAF BLADE Unit of measure: cm See Leaf Length (Character No. 12) on how LENGTH Values: average of 3 plants to collect leaves. The measurement does not The length of the leaf S25 = 14.6 cm for 5^(th) node include Petiole Length (Character No. 15). In blade from the base of the in 2001 in San Angelo some leaves the blade on one side of the petiole leaf blade to the apex of nursery = starts before the other side. This measure the leaf from the 5^(th), 10^(th), 14.1 cm for 10^(th) node should start from the lowest blade side. There and 15^(th) nodes in 2001 in San Angelo are leaves that have enations where a blade nursery = starts and then stops. The enations are not 12.1 cm for 15^(th) node considered part of the leaf blade because they in 2001 in San Angelo are very irregular from plant to plant and nursery within a plant. Distribution within Sesaco Comments: see Leaf Length (Character No. for 5^(th) leaf based on stable 12) for effects of environment lines in the crossing program The largest leaves are on photosensitive in 2001 (Total number of lines which when planted in the tropics fall into samples tested = 100) the 2^(nd) and 3^(rd) class low = 10.0 cm high = 31.0 cm 1 = <14.2 cm; 30% 2 = <18.4 cm; 43% 3 = <22.6 cm; 22% 4 = <26.8 cm; 2% 5 = >26.7 cm; 3% Distribution within Sesaco for 10^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 100) low = 9.8 cm high = 30.1 cm 1 = <13.9 cm; 30% 2 = <17.9 cm; 43% 3 = <22.0 cm; 19% 4 = <26.0 cm; 6% 5 = >25.9 cm; 2% Distribution within Sesaco for 15^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 59) low = 8.5 cm high = 27.5 cm 1 = <12.3 cm; 28.8% 2 = <16.1 cm; 42.4% 3 = <19.9 cm; 20.3% 4 = <23.7 cm; 5.1% 5 = >23.6 cm; 3.4% (14) LEAF BLADE Unit of measure: cm See Leaf Length (Character No. 12) on how WIDTH Values: average of 3 plants to collect leaves. There are many leaves that The width of the leaf S25 = 8.3 cm for 5^(th) node in are not symmetrical with lobbing on one side blade measured across the 2001 in San Angelo nursery = and not the other. The width should still be leaf blade at the widest 3.8 cm for 10^(th) node measured across the widest point on a line point at the 5^(th), 10^(th), and in 2001 in San Angelo perpendicular to the main vein of the leaf. 15^(th) nodes nursery = On some lines the width exceeds the length, 2.3 cm for 15^(th) node particularly on lobed leaves. in 2001 in San Angelo Comments: see Leaf Length (Character No. nursery 12) for effects of environment Distribution within Sesaco The largest leaves are on photosensitive for 5^(th) leaf based on stable lines which when planted in the tropics fall into lines in the crossing program the 2^(nd) and 3^(rd) class. The widest leaves are in 2001 (Total number of lobed. Normally, the leaves have turned from samples tested = 100) lobed to lanceolate by the 10^(th) leaf with the low = 2.3 cm exception of the tropical lines. high = 46.0 cm 1 = <11.0 cm; 52% 2 = <19.8 cm; 36% 3 = <28.5 cm; 10% 4 = <37.3 cm; 1% 5 = >37.4 cm; 1% Distribution within Sesaco for 10^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 100) low = 1.8 cm high = 37.0 cm 1 = <8.8 cm; 91% 2 = <15.5 cm; 6% 3 = <22.9 cm; 1% 4 = <29.9 cm; 1% 5 = >29.8 cm; 1% Distribution within Sesaco for 15^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 59) low = 1.2 cm high = 33.0 cm 1 = <7.6 cm; 94.9% 2 = <13.9 cm; 3.4% 3 = <20.3 cm; 0% 4 = <26.6 cm; 0% 5 = >26.5 cm; 1.7% (15) PETIOLE Unit of measure: cm See Leaf Blade Length (Character No. 13) LENGTH Values: average of 3 plants on how to collect leaves. In some leaves, the The length of the petiole S25 = 8.0 cm for 5^(th) node in blade on one side of the petiole starts before from the base of the 2001 in San Angelo nursery the other side. This measure should end where petiole to the start of the 4.6 cm for 10^(th) node in the earliest blade starts. There are leaves that leaf blade at the 5^(th), 10^(th), 2001 in San Angelo nursery have enations where a blade starts and then and 15^(th) nodes 2.7 cm for 15^(th) node in stops. The enations are not considered part of 2001 in San Angelo nursery the leaf blade because they are very irregular Distribution within Sesaco from plant to plant and within a plant and for 5^(th) leaf based on stable should be measured as part of the petiole. lines in the crossing program Comments: see Leaf Length (Character No. in 2001 (Total number of 12) for effects of environment samples tested = 100) The largest leaves are on photosensitive low = 3.7 cm lines which when planted in the tropics fall into high = 27.5 cm the 2^(nd) and 3^(rd) class 1 = <8.4 cm; 40% 2 = <13.2 cm; 47% 3 = <18.0 cm; 10% 4 = <22.7 cm; 2% 5 = >22.6 cm; 1% Distribution within Sesaco for 10^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 100) low = 1.4 cm high = 28.5 cm 1 = <6.8 cm; 77% 2 = <12.2 cm; 20% 3 = <17.7 cm; 1% 4 = <23.1 cm; 1% 5 = >23.0 cm; 1% Distribution within Sesaco for 15^(th) leaf based on stable lines in the crossing program in 2001 (Total number of samples tested = 59) low = 1.4 cm high = 20.0 cm 1 = <5.1 cm; 83.1% 2 = <8.8 cm; 13.6% 3 = <12.6 cm; 1.7% 4 = <16.3 cm; 0% 5 = >16.2 cm; 1.7% (16) NUMBER OF Unit of measure: Actual The rating can be taken from about 60 days CARPELS PER number after planting to all the way to the end of the CAPSULE Values: crop. The predominant number 2 = bicarpellate There are many plants with mixed number of carpels per capsule in 3 = tricarpellate of carpels as follows: the middle half of the 4 = quadricarpellate 1. Some bicarpellate plants will have one or capsule zone S25 = 2 for all crops in all more nodes near the center of the capsule zone nurseries that have tri- and/or quadricarpellate capsules Distribution within Sesaco and vice versa. based on the introductions 2. Most tri- and quadri-carpellate plants will received in 1982-2001 begin and end with bicarpellate nodes. (Total number of samples 3. Some plants have only one carpel that tested = 2702) develops. These capsules are generally bent, 2 = 97.6% but on examination the 2^(nd) carpel can be seen. 3 = 0.0004% 4. On all types, flowers may coalesce and 4 = 2.3% double or triple the number of carpels. Sesaco has not developed lines with more than 2 carpels. (17) CAPSULE Unit of measure: cm After the plants are physiologically mature, LENGTH FROM Values: taken on the median take 2 capsules from five plants from the 10cap TEST capsule in a 10 capsule middle of the capsule zone. On three capsule The length of the capsule sample per leaf axil lines, one central capsule and one from the bottom of the S25 = 2.7 cm in 2000 in axillary capsule should be taken from the same seed chamber to the top of Uvalde nursery = leaf axil. The measurement is taken on the the seed chamber from the 2.8 cm in 2001 in median capsule of single capsule lines and on outside of the capsule. Uvalde nursery the median central capsule on three capsule The tip of the capsule is Distribution within Sesaco lines. The measurement is taken on dry not included in the based on 10cap test in all capsules because the length can shorten as measurement. nurseries in 1997-2001 much as one mm on drydown. (Total number of samples The 10 capsules can be sampled from tested = 3145) physiological maturity through complete low = 1.3 cm drydown without an effect on this character. high = 4.5 cm Generally, the capsules in the middle of the 1 = <1.94 cm; 3.6% capsule zone are the longest on the plant. 2 = <2.58 cm; 67.4% Comments: the length of the capsule is 3 = <3.22 cm; 27.1% dependent on the amount of moisture, fertility, 4 = <3.86 cm; 1.7% and population. Higher moisture and fertility 5 = >3.85 cm; 0.3% increase the length. Higher population decreases the length even with adequate moisture/fertility. (18) SEED WEIGHT Unit of measure: grams See Capsule Length from 10cap Test PER CAPSULE Values: average of 10 (Character No. 17) for collection of capsules. FROM 10cap TEST capsules The capsules should be dried, the seed threshed The weight of the seed in S25 = 0.292 g in 2000 in out, and the seed weighed. a capsule from the center Uvalde nursery = The 10 capsules can be sampled from of the capsule zone 0.213 g in 2001 in physiological maturity through complete Uvalde nursery drydown without an effect on this character. Distribution within Sesaco After drydown, only capsules with all their based on 10cap test in all seed are taken. Thus, this test cannot be done nurseries in 1997-2001 on shattering lines after drydown. (Total number of samples Generally, the capsules in the middle of the tested = 3208) capsule zone have the highest seed weight per low = 0.053 g capsule on the plant. high = 0.476 g Comments: weight is dependent uon the 1 = <0.138 g; 2.7% amount of moisture, fertility, and population. 2 = <0.222 g; 51.1% Higher moisture and fertility increase the 3 = <0.307 g; 44.9% weight. Higher population decreases the 4 = <0.391 g; 1.2% weight even with adequate moisture/fertility. 5 = >0.390 g; 0.1% (19) CAPSULE Unit of measure: grams See Capsule Length from 10cap Test WEIGHT PER Values: average of 10 (Character No. 17) for collection of capsules. CAPSULE FROM capsules The capsules should be dried, the seed threshed 10cap TEST S25 = 0.108 g in 2000 in out, and the capsules weighed. The weight of the capsule Uvalde nursery = The 10 capsules can be sampled from from the center of the 0.138 g in 2001 in physiological maturity through complete capsule zone after the Uvalde nursery drydown without an effect on this character. seed has been removed Distribution within Sesaco Generally, the capsules in the middle of the based on 10cap test in all capsule zone have the highest capsule weight nurseries in 1997-2001 per capsule on the plant. (Total number of samples Comments: see Seed Weight per Capsule tested = 3207) from 10cap Test (Character No. 18) for the low = 0.059 g effects of environmental factors high = 0.395 g 1 = <0.126 g; 28.2% 2 = <0.193 g; 61.6% 3 = <0.261 g; 9.0% 4 = <0.328 g; 0.8% 5 = >0.327 g; 0.3% (20) CAPSULE Unit of measure: grams The weight is derived by dividing the WEIGHT PER CM Values: average of 10 Capsule Weight per Capsule from 10cap Test OF CAPSULE capsules (Character No. 19) by the Capsule Length from The weight of a capsule S25 = 0.040 g in 2000 in 10cap Test (Character No. 17). per cm of capsule from Uvalde nursery = The 10 capsules can be sampled from the center of the capsule 0.048 g in 2001 in physiological maturity through complete zone Uvalde nursery drydown without an effect on this character. Distribution within Sesaco Comments: this character is used instead of based on 10cap test in all capsule width. Capsule width is difficult to nurseries in 1997-2001 measure because there are so many variables in (Total number of samples a capsule. In a bicarpellate capsule, the width tested = 3144) differs when measuring across one carpel or low = 0.027 g both carpels. Capsules can also vary through high = 0.123 g the length of the capsule by being substantially 1 = <0.046 g; 11.5% narrower at the bottom, middle or top of the 2 = <0.065 g; 47.6% capsule. In 1997, four widths were measured 3 = <0.085 g; 35.6% on each capsule and then averaged. This 4 = <0.104 g; 4.8% average had a very high correlation to the 5 = >0.103 g; 0.5% capsule weight per cm of capsule. See Capsule Length from 10cap Test (Character No. 17) for effects of environmental factors (21) VISUAL SEED Subjective rating When the plants in a plot are dry below RETENTION Values: where the cutter bar would hit the plant, assign Amount of seed in most of Seed Retention a rating based on the following guidelines. If the capsules in the middle 8 = 100% just identifying lines for further testing, use half of the capsule zone 6 = 75% V/X/W ratings. If identifying lines to use in a when the plant(s) are dry 4 = 50% crossing program to improve seed retention, enough for direct harvest 2 = 25% use 0-8 ratings. Ratings 6-8 can be seen with a combine 0 = 0% without removing the capsule from the plant. Intermediate values can be For the other ratings, the capsules must be used. opened. Rating is an overall subjective number V = >74% seed retention since retention can vary from plant to plant and (sufficient seed retention for even within a plant. 10cap testing) The effects of the environment are not fully X = <75% seed retention known. There are indications that in drought (unsuitable for direct harvest) or very low fertility conditions, there is less W = >75% seed retention seed retention. When high propulations lead to on weathering in field after low moisture or fertility, there is less seed rains and/or winds for more retention. From normal conditions through than 3 weeks following high moisture/fertility conditions, there does complete drydown not appear to be an appreciable difference. S25 = W for all crops in all nurseries (22) SHAKER Unit of measure: Actual See Capsule Length from 10cap Test SHATTER Number expressed as (Character No. 17) for collection of capsules. RESISTANCE percentage The capsules should be dried. The capsules FROM 10cap TEST Values: average of 10 should then be placed in flasks on a reciprocal The amount of seed reten- capsules shaker with a 3.8 cm stroke with 250 tion after the capsules are S25 = 79% in 2000 in strokes/min for 10 minutes (see U.S. Pat. dry, inverted, and put Uvalde nursery = No. 6,100,452). The seed that comes out of the through a shaker 76% in 2001 in capsules should be weighed as ‘out seed.’ The Uvalde nursery retained seed should be threshed out of the Distribution within Sesaco capsules and weighed to compute the ‘total based on 10cap test in all seed’. The shaker shatter resistance is nurseries in 1997-2001 computed as a percentage as follows: (total (Total number of samples seed-out seed)/total seed. tested = 3043) The 10 capsules can be sampled from low = 0 physiological maturity through complete high = 100 drydown without an effect on this character for 1 = <20; 12.3% shatter resistant types. When taking capsules 2 = <40; 9.1% after drydown, only capsules with all their seed 3 = <60; 25.1% are taken. Thus, this test cannot be done on 4 = <80; 44.8% shattering lines after drydown. 5 = >79.9; 8.8% Comments: there are indications that in drought or very low fertility condition, there is less seed retention. When high populations lead to low moisture or fertility, there is less seed retention. From normal conditions through high moisture/fertility conditions, there does not appear to be an appreciable difference in seed retention. Lines with shaker shatter resistance >64.9% are known as non-dehiscent lines (see U.S. Pat. No. 6,100,452). (23) CAPSULE Subjective rating The rating is based on visual seed retention SHATTERING Values: and other visual observations. The plants TYPE SUS = Super-shattering (<2 remain standing in the field without shocking. Amount of seed retention visual seed retention- GS plants can be identified while the plant in a line or plant equates to <25%) is putting on capsules or at drydown because SHA = Shattering (<4 visual the carpels in the capsules do not form false seed retention-equates to membranes. There are plants that will have <50%) capsules with false membranes on the lower SSH = Semi-shattering (4-6 and upper nodes but most of the capsules show visual seed retention- no false membranes. equates to 50 to 75%) ID plants can be identified during the SR = Shatter resistant (>6 growing season in that they have enations on visual seed retention without the bottoms of the leaves. At dry down they are id or gs alleles-equates to more difficult to distinguish from other lines >75%) that have closed capsules (other than GS). ID = Indehiscent (presence There is less of a suture than other capsule of id/id with capsule closed) types. IDO = Indehiscent (presence SUS, SHA, SSH, and SR are defined by of id/id with capsule open at Visual Seed Retention (Character No. 21). tip) Comments: most environmental factors do GS = Seamless (presence of not have much of an effect on capsule gs/gs with capsule closed) shattering type other than to make it more GSO = Seamless (presence of difficult to distinguish in the overlab zone. gs/gs with capsule open at Generally, higher moisture, higher fertility, and tip) lower populations will decrease the shattering a S25 = SR in all crops in all small amount-less than 10%. nurseries The wind can have a large effect in decreasing the amount of seed retention. Rain, dew and fog can also reduce seed retention. (24) NON-DEHISCENT Rating: Lines are designated as ND only after they TEST Values: have undergone a minimum of 3 shaker shatter A line that has passed the ND = Non-dehiscent line resistance tests. In order to be considered an non-dehiscent test of XX = Line that does not ND variety, the line must pass the ND having shaker shatter pass the non-dehiscent test threshold in multiple nurseries for multiple resistance >64.9%. ND S25 = ND for all crops in all years. For example, S25 has a mean of 72.9% lines should not have id or nurseries seed retention in 113 shaker shatter resistance gs alleles. Distribution within Sesaco tests for 1997-2001. based on 10cap test in all nurseries in 1997-2001 (Total number of samples tested = 3031 ND = 56.8% XX = 43.2% (25) DAYS TO Unit of measure: days This data is taken as a date and later FLOWERING Values: number of days converted to number of days. Flowering is Number of days from S25 = 37 days in 2000 in defined as flowers that are open-not buds. planting until 50% of the Uvalde nursery = This is a somewhat subjective unit of plants are flowering 36 days in 2001 in measure because there is little difference in the Uvalde nursery appearance of a line that has 40% and a line Distribution within Sesaco that has 60% of plants with flowers. In based on lines in Uvalde addition, the plots are not walked every day, nursery in 2000-2001 and thus there is an estimate that the plot was (Total number of samples 50% a few days before or after the date of data tested = 1831) collection. low = 33 days Comments: flowering can be accelerated high = 89 days by drought and it can be delayed by higher 1 = <44.2 days; 87.9% moisture and/or fertility. Higher heat units will 2 = <55.4 days; 7.8% decrease the days to flowering. 3 = <66.6 days; 2.4% Some lines are photosensitive and will only 4 = <77.8 days; 1.7% begin flowering at a certain number of hours of 5 = >77.7 days; 0.2% daylight. Start of flowering does not always equate to start of capsule formation. Many lines will flower and not set capsules from the first flowers. (26) DAYS TO Unit of measure: days This data is taken as a date and later FLOWER Values: number of days converted to number of days. Flowering is TERMINATION S25 = 75 days in 2000 in defined as flowers that are open-not buds. At Number of days from Uvalde nursery = the end of the flowering period, the rate that a planting until 90% of the 66 days in 2001 in plant puts on open flowers is reduced. Thus, plants have stopped Uvalde nursery there can be more than 10% of plants with buds flowering Distribution within Sesaco and still have reached this measure since there based on lines in Uvalde will not be more than 10% flowering any one nursery in 2000-2001 day. (Total number of samples This is a somewhat subjective unit of tested = 2668) measure because there is little difference in the low = 61 days appearance of a line that has 85% and a line high = 114 days that has 95% of plants with no flowers. In 1 = <71.6 days; 21.1% addition, the plots are not walked every day, 2 = <82.2 days; 61.5% and thus there is an estimate that the plot was 3 = <92.8 days; 15.9% 90% a few days before or after the date of data 4 = <103.4 days; 0.8% collection. Another problem is that under low 5 = >103.3 days; 0.8% moisture conditions the plots can reach the 90% mark only to begin flowering again after a rain. In those cases the data is adjusted to the later number of days. The measure is based on the number of plants and not the number of flowering heads. The branches will stop flowering before the main stem, and thus the plot will appear like there are more plants not flowering. Comments: flower termination can be accelerated by lower moisture and/or fertility, and it can be delayed by higher moisture and/or fertility. Higher heat units will decrease the Days to Flower Termination. It is known that there are lines that stop flowering sooner than expected in northern latitudes, but it is not known if this is due to shorter photoperiod or cool temperatures. (27) DAYS TO Unit of measure: days This data is taken as a date and later PHYSIOLOGICAL Values: number of days converted to number of days. Physiological MATURITY S25 = 87 days in 2000 in maturity (PM) is defined as the point at which Number of days from Uvalde nursery = ¾ of the capsules have seed with final color. planting until 50% of the 92 days in 2001 in In most lines, the seed will also have a seed plants reach physiological Uvalde nursery line and tip that are dark. maturity Distribution within Sesaco This is a somewhat subjective unit of based on lines in Uvalde measure because there is little difference in the nursery in 2000-2001 appearance of a line that has 40% and a line (Total number of samples that has 60% of plants with PM. In addition, tested = 2374) the plots are not walked every day, and thus low = 77 days there is an estimate that the plot was 50% a few high = 140 days days before or after the date of data collection. 1 = <89.6 days; 16.8% Comments: at physiological maturity, the 2 = 102.2 days; 58.0% seed will germinate. In addition, most of the 3 = <114.8 days; 23.6% seed will continue maturing enough for 4 = <127.4 days; 1.4% germination, but will not have as much seed 5 = >127.3 days; 0.2% weight. Since in even a fully mature plant, there is less seed weight made at the top of the plant, this loss of seed weight does not seriously affect the potential seed weight of the plant. Physiological maturity can be accelerated by lower moisture and/or fertility, and it can be delayed by higher moisture and/or fertility. Higher heat units will decrease the days to physiological maturity. Cool weather can delay physiological maturity. (28) SEED COLOR Subjective rating This data is taken in the laboratory with the The color of the seed coat Values: same lighting for all samples. The seed from WH = White the whole plant is used. BF = Buff Seed coat color is taken on mature seeds. If TN = Tan there is any abnormal termination, the colors LBR = Light brown are not quite as even. The color of immature GO = Gold seed varies. Usually light seeded lines have tan LGR = Light gray to light brown immature seed; tan, light brown, GR = Gray gold, brown light gray, and gray lines have BR = Brown lighter immature seed; black lines can have tan, RBR = Reddish brown brown, or gray immature seed. BL = Black Usually, moisture, fertility, population and S25 = BF in all crops in all light intensity do not have an effect on seed nurseries coat color. Seeds from lines in a drought may Distribution within Sesaco have a yellowish tinge. Seeds in some lines in based on seed harvested in all the tan, light brown and gold range can change nurseries in 1982-2001 (Total from year to year among themselves. number of samples tested = 161,809) WH = 0.8% BF = 74.8% TN = 9.0% LBR = 1.4% GO = 1.5% LGR = 0.6% GR = 1.4% BR = 6.5% RBR = 0.6% BL = 3.5% (29) SEED WEIGHT- Unit of measure: grams Count out 100 seeds and weigh. The seed must 100 SEEDS FROM Values: average of 3 samples be dry. WHOLE PLANT S25 = 0.25 g in 2000 in Comments: the weight increases with Weight of 100 seeds taken Uvalde nursery = higher moisture/fertility. Generally, the weight from the whole plant 0.27 g in 2001 in is lighter than the seed weight taken from the Uvalde nursery 10cap test. Distribution within Sesaco based on stable lines in all nurseries in 1997-2001 (Total number of samples tested = 1984) low = 0.10 g high = 0.46 g 1 = <0.172 g; 0.6% 2 = <0.244 g; 10.1% 3 = <0.316 g; 56.0% 4 = <0.388 g; 26.1% 5 = >0.387 g; 7.3% (30) UVALDE KILL Subjective rating On the week a plot reaches PM, a rating is RESISTANCE Values: ratings are based on assigned. The ratings are then taken for 2 The amount of plants the number of plants killed in additional weeks. The three ratings are killed by root rots in the a plot. Before physiological averaged for a final kill rating. For example, if Sesaco nurseries in maturity (PM), the following a plot has a final kill of 766, the average for the Uvalde, TX ratings are used: plot will be 6.33. When a value of 1 or 2 is 1 = >90% kill before Days to assigned, there are no additional ratings and Flowering Termination there is no averaging. (Character No. 26) There are three root diseases that affect 2 = >90% kill between Days sesame in Texas: Fusarium oxysporum, to Flowering Termination Macrophomina phaseoli, and Phytophtora (Character No. 26) and Days parasitica. Between 1988 and the present, to Physiological Maturity spores of these three have been accumulated in (Character No. 27) one small area of Uvalde, and thus it is an After PM, the following excellent screening area for the diseases. ratings are used: Although each root rot attacks sesame in a 3 = >90% kill different way with different symptoms, no 4 = 50 to 89% kill effort is made to differentiate which disease is 5 = 25 to 49% kill the culprit in each plot. Pathological screenings 6 = 10 to 24% kill in the past have found all 3 pathogens present 7 = less than 10% kill in dead plants. 8 = no kill Comments: normally, the ratings will S25 = 5.67 in 2000 in decrease a maximum of one value per week. Uvalde nursery = There is an overlap between any two ratings, 6.22 in 2001 in but this is overcome to a certain extent by Uvalde nursery using three ratings over 2 weeks. Distribution within Sesaco The amount of kill is usually increased with based on lines in Uvalde any type of stress to the plants. Drought can nursery in 2000-2001 increase the amount of Macrophomina; too (Total number of samples much water can increase the amount of tested = 3045) Phytophtora; high temperatures and humidity low = 1.00 can increase the amount of Fusarium and high = 8.00 Phytophtora. High population can increase all 1 = <1.6; 1.7% three diseases. 2 = <3.2; 16.7% 3 = <4.8; 38.7% 4 = <6.4; 31.2% 5 = >6.3; 11.6% (31) RESISTANCE TO Subjective rating Ratings can be done in several ways: FUSARIUM WILT Values: 0 to 8 scale 1. Take ratings after the disease is no longer (F. oxysporum) % of infected plants increasing Amount of resistance to 8 = Zero disease 2. Take ratings on consecutive weeks until Fusarium 7 = <10% infected disease is no longer increasing and average 4 = 50% infected ratings. 1 = >90% infected 3. Take periodic ratings and average ratings. 0 = all infected Comments: Fusarium has been a problem NT = no data in South Texas, particularly on fields that have NEC = no economic been planted with sesame before. Normally, damage-not enough disease only the Uvalde Kill Resistance (Character No. to do ratings 30) rating is taken. S25 = 6 in 1998-2001 in Uvalde nursery (32) RESISTANCE TO Subjective rating See Methodology for Fusarium PHYTOPHTORA See Values for Fusarium Comments: Phytophtora has been a STEM ROT (P. S25 = NT problem in Arizona and Texas, particularly on parasitica) fields that have been over-irrigated. Normally, Amount of resistance to only the Uvalde Kill Resistance (Character No. Phytophtora. 30) rating is taken. (33) RESISTANCE TO Subjective rating See Methodology for Fusarium CHARCOAL ROT See Values for Fusarium Comments: Macrophomina has been a (Macrophomina S25 = NT problem in Arizona and Texas, particularly on phaseoli) fields that go into a drought. Normally, only Amount of resistance to the Uvalde Kill Resistance (Character No. 30) Charcoal rot rating is taken. (34) RESISTANCE TO Subjective rating See Methodology for Fusarium BACTERIAL See Values for Fusarium Comments: this disease occurs occasionally BLACK ROT S25 = NEC for 1997 in when there is continual rainy weather with few (Pseudomonas Oklahoma nursery^(c) clouds. In most years, the disease abates once sesami) the weather changes. No economic damage has Amount of resistance to been noticed. Pseudomonas (35) RESISTANCE TO Subjective rating Ratings can be done in several ways: WHITE FLY Values: 0 to 8 scale 1. Take ratings after the insects are no longer (Bemisia 8 = Zero insects increasing. argentifolii) 7 = Few insects 2. Take ratings on consecutive weeks until Amount of resistance to 4 = Many insects insects are no longer increasing and average the silverleaf white fly 1 = Insects killing the plants ratings. NT = no data 3. Take periodic ratings and average ratings. NEC = no economic Comments: there have been very few years damage-not enough insects (1991-1995) where the incidence of silverleaf to do ratings white fly has affected nurseries or commercial S25 = NEC in 2000 in crops. In most years, a few white flies can be Uvalde nursery seen in the sesame with no economic damage. Higher temperatures decrease the number of days between generations. There are indications that higher moisture and fertility increase the incidence of white flies, but there is no definitive data. (36) RESISTANCE TO Subjective rating See Methodology for White Fly GREEN PEACH See Values for White Fly Comments: there have been very few years APHIDS (Myzus S25 = NEC in 2000 in (1990-1995) where the incidence of green persicae) Uvalde nursery peach aphid has affected nurseries or Amount of resistance to commercial crops. In most years, a few aphids the green peach aphid can be seen in the sesame with no economic damage. There have been many years in West Texas when the cotton aphid has decimated the cotton and did not build up on adjacent sesame fields. Higher moisture and fertility increase the susceptibility to aphids. (37) RESISTANCE TO Subjective rating See Methodology for White Fly POD BORERS See Values for White Fly Comments: there have been very few years (Heliothis spp.) S25 = NEC in 2001 in (1985) where the incidence of Heliothis has Amount of resistance to Uvalde nursery affected nurseries or commercial crops. In most pod borers years, a few borers can be seen in the sesame with no economic damage. (38) RESISTANCE TO Subjective rating See Methodology for White Fly ARMY WORMS See Values for White Fly Comments: there have been very few years (Spodoptera spp.) S25 = NT (1984-1987) where the incidence of Amount of resistance to Spodoptera has affected commercial crops in army worms Arizona. In Texas, army worms have decimated cotton and alfalfa fields next to sesame without any damage to the sesame. It is not known if the Arizona army worm is different from the Texas army worm. (39) RESISTANCE TO Subjective rating See Methodology for White Fly CABBAGE See Values for White Fly Comments: there have been very few years LOOPERS (Pieris S25 = NT (1992-1993) where the incidence of cabbage rapae) loopers has affected nurseries. In commercial Amount of resistance to sesame, cabbage loopers have been observed cabbage loopers with no economic damage. # 1988 to 1997 and on 76 cm beds from 1998 to 2001. The nursery was pre-irrigated and has had 2-3 post-plant irrigations depending on rainfall. The fertility has varied from 30-60 units of nitrogen. # 101 cm beds. The nursery was pre-irrigated in years when there was no planting rain and has had 0 to 1 post-plant irrigations depending on rainfall. The fertility has varied from 20-50 units of nitrogen. # nitrogen.

[0051] In developing sesame varieties for the United States, there are four major characters that are critical: Shaker Shatter Resistance (Character No. 22), Uvalde Kill Resistance (Character No. 30), Days to Physiological Maturity (Character No. 27), and Seed Weight—100 Seeds from Whole Plant (Character No. 29). The first three characters contribute to yield which is the ultimate determinant for the farmer to grow a variety. In improving the characters, the yields have to be comparable to or better than current varieties, or provide a beneficial improvement for a particular geographical or market niche. Shaker Shatter Resistance determines how well the plants will retain the seed while they are drying down in adverse weather. Uvalde Kill Resistance determines whether the plants can finish their cycle and have the optimum seed fill. Days to Physiological Maturity determines how far north and to which elevation the varieties can be grown. Seed Weight—100 Seeds from Whole Plant determines the market for the seed. Lack of Uvalde Kill Resistance can reduce Seed Weight—100 Seeds from Whole Plant.

[0052] There are other characters important in developing commercial sesame varieties explained in Langham, D. R. and T. Wiemers, 2002, “Progress in mechanizing sesame in the US through breeding”, In: J. Janick (ed.), Trends in new crops and new uses, ASHS Press, Alexandria, Va. Branching Style (Character No. 1), Height of Plant (Character No. 5) and Height of First Capsule (Character No. 6) are important in combining. Capsule Zone Length (Character No. 7), Number of Capsule Nodes (Character No. 8), Average Internode Length within Capsule Zone (Character No. 9), Resistance to Drought (Character No. 11), and Seed Weight per Capsule (Character No. 18) are important in creating potential Yield at Drydown (Character No. 10). Leaf dimensions (Characters No. 12, 13, 14, and 15) are important in determining optimum populations. Number of Carpels per Capsule (Character No. 16), Capsule Length (Character No. 17), Capsule Weight per Capsule (Character No. 19), and Capsule Weight per cm of Capsule (Character No. 20) are important in breeding for Visual Seed Retention (Character No. 21) which leads to testing for Shaker Shatter Resistance and determining the Capsule Shattering Type (Character No. 23). Days to Flowering (Character No. 25), Days to Flower Termination (Character No. 26), and Days to Physiological Maturity (Character No. 27) are highly correlated and important in determining the geographical range for the variety. In the United States and Europe, the Seed Color (Character No. 28) is important since the majority of the market requires white or buff seed. There are limited markets for gold and black seed in the Far East. All other colors can only be used in the oil market. In the United States, resistance to diseases and pests (Characters No. 31 to 39) are critical to allow the crop to reach maturity and harvest.

[0053]FIG. 2 provides the Shaker Shatter Resistance of all the varieties released by Sesaco since 1981. The figures are the mean from all testing in all nurseries from 1997 to 2001. S01 was an indehiscent variety with excellent seed retention, but the seed could not be threshed out without making the seed unmarketable. S02 through S10, S12, and S14 were developed for swathing at Days to Physiological Maturity, drying in windrows, and then picking up with a combine. All of these varieties had good yields when there was little rain and high temperatures at harvest time. In the bad weather, the yields were reduced. Attempts were made to let these varieties dry down standing and then combining, but the yields were not commercially adequate. S11 was the first variety that could be left standing for harvest with adequate yields in normal weather. With the exception of S17, varieties S15 through S22 were released for specific niches. S17 replaced S11 in most locations until it was replaced by S23 and S24. S23 is considered to be the minimum acceptable Shaker Shatter Resistance for commercial use. S25 provides about 73% seed retention, a desirable amount for mechanized harvesting.

[0054]FIG. 3 provides the Uvalde Kill Resistance of all the varieties released by Sesaco since 1981 as compared in the Uvalde nursery in 2001 using all plots. Uvalde Kill Resistance is a composite rating of resistance to three root rots: Fusarium, Phytophtora, and Macrophomina. When sesame is first introduced into a growing area, there are few disease problems, but over time the spores of these fungi accumulate and disease resistance becomes critical. When sesame was first introduced in Uvalde in 1988, the yields were high. As farmers planted on the same fields in subsequent years, the yields decreased. S11 was very susceptible to these root rots, and thus, it was replaced by S17, which was subsequently replaced by S23 and S24. S25 exhibits a rating of 6.72, which is better than previously released non-dehiscent lines.

[0055]FIG. 4 provides the mean Days to Physiological Maturity of all the varieties over the past four years in the Uvalde nursery. In the United States, sesame is currently grown from South Texas to southern Kansas. The growing window of a crop is determined by the earliest the crop can be planted in the spring as the ground warms up, and the onset of cold weather in the fall. Current sesame varieties require about 21° C. ground temperature to establish an adequate population. In most years, the ground is warm enough in South Texas in middle April and in southern Kansas in late May. Current sesame varieties require night temperatures above 5° C. for normal termination. In most years, the night temperatures are warm enough in South Texas until middle November and in southern Kansas until middle October. There have been years when cold fronts affect the growth of sesame in the middle of September in the north. East of Lubbock, Tex., the elevations begin climbing towards the Rocky Mountains, and there are later warm temperatures in the spring and earlier cold temperatures in the fall. In all years, if the sesame is planted as early as temperatures allow, lines with Days to Physiological Maturity of 105 days or less will have no problems. However, most areas are rainfed, and it is essential to have a planting rain before planting the sesame. Thus, the earlier the Days to Physiological Maturity of the variety, the more flexibility the farmers have with planting date. In South Texas, the goal is to have varieties with a Days to Physiological Maturity of less than 110 days while in southern Kansas the goal is less than 90 days. The mean Days to Physiological Maturity for S25 is 90.25 days.

[0056]FIG. 5 provides the average Seed Weight—100 Seeds from Whole Plant of all varieties between 1997 and 2001. The dehulled market is the premium use of sesame in the United States and Europe. In recent years, dehulled processors have been increasing the specifications of Seed Weight—100 Seeds from Whole Plant to between 0.28 and 0.30 g, and larger seed is preferable. To date, the Sesaco varieties with the highest Seed Weight—100 Seeds from Whole Plant have had marginal Shaker Shatter Resistance and poor Uvalde Kill Resistance. Some markets have no specifications on seed weight, but larger seed is still desirable. The mean Seed Weight—100 Seeds from Whole Plant for S25 is 0.29 g, which falls within the desired specifications.

[0057] Prior to 1988, all of the commercial sesame in the United States was grown in Arizona. S01 through S10, S12, and S14 were specifically developed for Arizona. From 1988 to 1991, there was a transition from Arizona to Texas. In 1996, sesame spread into Oklahoma, and in 1998, into Kansas. In introducing sesame to Texas, the swathing technology was tried on S07 and S10, but farmers did not have the proper equipment; the plants fell into the furrows and could not be picked up; and/or blowing sand covered parts of the windrows. S11 was the first variety that could be combined directly, and S11 persisted until 1995. However, in addition to being susceptible to root rots, S11 was also susceptible to pests such as green peach aphids (Myzus persicae) (Character No. 36) and silverleaf white fly (Bemisia argentifolii) (Character No. 35), and S11 had too long a Days to Physiological Maturity (109 days) for West Texas. S15 was introduced for West Texas, but shorter Days to Physiological Maturity (107 days) was still not early enough and the Shaker Shatter Resistance (38.7% seed retention) was not adequate. S16 was released specifically because it was tolerant to the white fly, but it did not have good Shaker Shatter Resistance (42% seed retention) or Uvalde Kill Resistance (2.0 rating), and it was susceptible to lodging. S17 had better Uvalde Kill Resistance (5.56 rating), aphid resistance, and white fly resistance than S11. With an earlier Days to Physiological Maturity (98.5 days), S17 was the first variety that was suitable for West Texas and southern Kansas; however, S17 was susceptible to lodging. S18 and S21 were released for flavor for the Japanese organic market. S19 had improved lodging resistance over S17, but the yields in non-windy areas were not as good as S17. S20 and S22 were released because of excellent drought resistance. In the rainfed areas of Central Texas, the yields of S20 and S22 were higher than S17, the large seed was desirable to processors, and no root rots had been seen. However, the root rot spores began accumulating, and the S20 and S22 had to be discontinued. S24 replaced S17 based on higher Shaker Shatter Resistance (74.4% for S24 compared to 60.8% for S117), better Uvalde Kill Resistance (rating of 6.18 for S24 compared to 5.56 for S17), shorter Days to Physiological Maturity (95.0 days for S24 compared to 98.5 for S17), and larger Seed Weight—100 Seeds from Whole Plant (0.30 g for S24 compared to 0.26 g for S17). S23 was used in southern Kansas and northern Oklahoma because of a shorter Days to Physiological Maturity than S24 (89 days for S23 compared to 95 days for S24), but the Seed Weight—100 Seeds from Whole Plant was marginal (0.26 g for S23 compared to 0.30 g for S24).

[0058] S25 was developed to replace S23 in southern Kansas and northern Oklahoma even though the Days to Physiological Maturity is not quite as short (90.25 days for S25 compared to 89.0 days for S23). Compared to S23, S25 has better Shaker Shatter Resistance (72.9% seed retention for S25 compared to 65.7% seed retention for S23) and Uvalde Kill Resistance (rating of 6.72 for S25 compared to 6.22 for S23), but the major reason for the replacement is the Seed Weight—100 Seeds from Whole Plant (0.29 g for S25 compared to 0.26 g for S23). S25 will also be used to penetrate new growing areas in West Texas in higher elevations.

[0059] Over the past 24 years, Sesaco has tested 2,738 introductions from all over the world. Commercial samples have been obtained from China, India, Sudan, Ethiopia, Burkina Faso, Nigeria, Mozambique, Pakistan, Myanmar, Bangladesh, Vietnam, Egypt, Mexico, Guatemala, Nicaragua, Venezuela, Thailand, Turkey, Upper Volta, Uganda, Mali, Kenya, Indonesia, Sri Lanka, Afghanistan, Philippines, Colombia, Ivory Coast, Gambia, Somalia, Eritrea, Paraguay, and El Salvador. Additional research seed has been received from the commercial countries and from many other countries such as Australia, Iraq, Iran, Japan, Russia, Jordan, Yemen, Syria, Morocco, Saudi Arabia, Angola, Argentina, Peru, Brazil, Cambodia, Laos, Sri Lanka, Ghana, Gabon, Greece, Italy, South Korea, Libya, Nepal, Zaire, and Tanzania. Research seed received from one country may have originated from another unspecified country. All the commercial and research introductions have Capsule Shattering Type “SHA”. Using selected characters from Table I, Table II provides a character differentiation between S25 and name cultivars from all over the world. TABLE II Character Differentiation of Various Sesame Varieties^(a) Character Rating Name cultivars tested by Sesaco Capsule Shattering SHA From Venezuela: Venezuela 51, Type (Character No. Venezuela 52, Guacara, Aceitera, Inamar, 23) Acarigua, Morada, Capsula Larga, Arawaca, Piritu, Glauca, Turen, DV9, Fonucla, UCLA From Mexico: Pachequeno, Yori, Anna, Teras, Denisse, Canasta, Tehvantepeter From India: TMV1, TMV3 From Turkey: Ozberk, Muganli, Gamdibi, Marmara From Israel: DT45 From Guatemala: R198, R30 From Texas: Llano, Margo, Dulce, Blanco, Paloma, Oro, Renner 1 and 2, Early Russian From California: UCR3, UCR4, Eva, Calinda (Cal Beauty) From Thailand: KU18 From Korea: Danback, Gwansan, Pungyiong, Suweon, Yuseong, Hanseon, Ahnsan, Kwangsan, Jinback, Pungsan, Sodan, Yangheuk, Konheuk, Whaheuck, Sungboon SSH From Sesaco: S02, S03, S04, S05, S06, S07, S08, S09, S10, S12, S14 ID From Venezuela: G2, Morada id From Texas: Rio, Delco, Baco, Improved Baco, Roy, Eli From South Carolina: Palmetto From California: UCR234 From Sesaco: S01 SR All others, go to Non-dehiscent Test Non-dehiscent Test XX From Sesaco: S11, S15, S16, S17, S18, (Character No. 24) S19, S20, S21 ND All others, go to Branching Style Branching Style U From Sesaco: S22, 19A, 11W (Character No. 1) B All others, go to Number of Capsules per Leaf Axil Number of Capsules 3 From Sesaco: S23 per Leaf Axil 1 From Sesaco: S24, S25 (Character No. 2)

[0060] Table III compares S24 and S25 using all of the characters in Table I. In Table III, some of the values are different from the values used in FIGS. 2-5. The values in Table III are from side by side plots grown under the same conditions, and the values in FIG. 2 to FIG. 5 are based on averages of all of the S24 and S25 plots grown in the nurseries and dates indicated. The major differences are indicated by an asterisk in the “Dif” column. TABLE III Character Comparison of S25 and S24^(a) No. Character Year/nursery S24 S25 Dif 1 Branching Style All B B 2 Number of Capsules per Leaf Axil All 1 1 3 Maturity Class 1998-2001 UV M E * 4 Plant Phenotype All B1M B1E * 5 Height of Plant (cm) 2000 UV 126 125 2001 UV 129 126 6 Height of First Capsule (cm) 2000 UV 50 49 2001 UV 45 41 7 Capsule Zone Length (cm) 2000 UV 76 76 2001 UV 84 86 8 Number of Capsule Nodes 2000 UV 27 26 2001 UV 29 29 9 Average Internode Length within 2000 UV 2.8 3.0 Capsule Zone (cm) 2001 UV 2.9 3.0 10 Yield at Drydown (kg/ha) 2000 UV 1270 1343 2001 UV 1074 1614 * 2000 SA 202 87 * 11 Resistance to Drought 2000 SA 3.6 2.5 * 12 Leaf Length (cm)  5^(th)-2001 SA 19.7 22.6 10^(th)2001 SA 19.2 18.7 15^(th)-2001 SA 15.5 14.8 13 Leaf Blade Length (cm)  5^(th)-2001 SA 14.4 14.6 10^(th)-2001 SA 14.3 14.1 15^(th)-2001 SA 12.7 12.1 14 Leaf Blade Width (cm)  5^(th)-2001 SA 9.3 8.3 10^(th)-2001 SA 4.2 3.8 15^(th)-2001 SA 2.7 2.3 15 Petiole Length (cm)  5^(th)-2001 SA 5.3 8.0 10^(th)-2001 SA 4.9 4.6 15^(th)-2001 SA 2.8 2.7 16 Number of Carpels per Capsule All 2 2 17 Capsule Length (cm) 2000 UV 2.9 2.7 2001 UV 2.9 2.8 18 Seed Weight per Capsule (g) 2000 UV 0.186 0.202 * 2001 UV 0.198 0.213 * 19 Cansule Weight per Capsule (g) 2000 UV 0.118 0.108 2001 UV 0.157 0.138 20 Capsule Weight per cm of 2000 UV 0.041 0.040 Capsule (g) 2001 UV 0.053 0.048 21 Visual Shatter Resistance All W W 22 Shaker Shatter Resistance (%) 2000 UV 77 79 2001 UV 79 76 23 Capsule Shattering Type All SR SR 24 Non-dehiscent Test All ND ND 25 Days to Flowering 2000 UV 39 37 * 2001 UV 39 36 * 26 Days to Flower Termination 2000 UV 78 75 * 2001 UV 69 66 * 27 Days to Physiological Maturity 2000 UV 91 87 * 2001 UV 97 92 * 28 Seed Color All BF BF 29 Seed Weight - 100 Seeds from 2000 UV 0.25 0.25 Whole Plant (g) 2001 UV 0.27 0.27 30 Uvalde Kill Resistance 2000 UV 4.60 5.67 * 2001 UV 5.67 6.22 * 31 Resistance to Fusarium Wilt 1998-2001 UV 5 6 * (F. oxysporum) 32 Resistance to Phytophtora Stem Rot NT NT (P. parasitica) 33 Resistance to Charcoal Rot NT NT (Macrophomina phaseoli) 34 Resistance to Bacterial Black Rot 1997 OK NEC NEC (Pseudomonas sesami) 35 Resistance to Silverleaf White Fly 2000 UV NEC NEC (Bemisia argentifolii) 36 Resistance to Green Peach Aphid 2000 UV NEC NEC (Myzus persica) 37 Resistance to Pod Borer 2001 UV NEC NEC (Heliothis spp.) 38 Resistance to Army Worms NT NT (Spodoptera spp.) 39 Resistance to Cabbage Loopers NT NT (Pieris rapae)

[0061] Maturity Class, Plant Phenotype, Days to Flowering, Days to Flower Termination, and Days to Physiological Maturity are all related. S25 is earlier than S24 allowing S25 to penetrate further north and into higher elevations than S24.

[0062] Yield at Drydown in Uvalde, Seed Weight per Capsule, Uvalde Kill Resistance, and Resistance to Fusarium Wilt are all related. The Yield at Drydown and Seed Weight per Capsule of S24 was reduced by greater susceptibility to root rots than S25. In years when there is little disease, the Yield at Drydown and Seed Weight per Capsule of S24 and S25 are not statistically different.

[0063] Yield at Drydown in San Angelo and Drought Resistance are related. S25 is less resistant to drought than S24. In 2000 in San Angelo, there was a drought with very low moisture in the soil profile at planting, and the one irrigation of less than 50 mm was applied too late to help the crop. S25 is not recommended for rainfed conditions where the annual rainfall is less than 700 mm per year or where there is a drought in progress.

[0064] On Apr. 29, 2002, a deposit of at least 2500 seeds of sesame plant S25 was made by Sesaco Corporation under the provisions of the Budapest Treaty with the American Type Culture Collection (ATCC), 10801 University Boulevard, Manassas, Va. 20110-2209, and the deposit was given ATCC Accession No. ______. This deposit will be maintained in the ATCC depository for a period of 30 years or 5 years after the last request or for the enforceable life of the patent, whichever is longer. Should the seeds from the sesame line S25 deposited with the American Type Culture Collection become inviable, the deposit will be replaced by Sesaco Corporation upon request.

[0065] The foregoing invention has been described in some detail by way of illustration and characters for purposes of clarity and understanding. However, it will be obvious that certain changes and modifications may be practiced within the scope of the invention as limited only by the scope of the appended claims. 

I claim:
 1. Seed of a sesame plant S25, a sample of said seed having been deposited under ATCC Accession No. ______.
 2. A sesame plant or its parts produced by growing the seed of sesame plant S25, a sample of said seed having been deposited under ATCC Accession No. ______.
 3. Pollen of said sesame plant of claim
 2. 4. A sesame plant having all the physiological and morphological characteristics of sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.
 5. A sesame plant having all the physiological and morphological characteristics of a sesame plant produced by growing the seed having been deposited under ATCC Accession No. ______.
 6. A sesame plant or its parts having as a parent sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.
 7. Seed from a progeny sesame plant having as a parent sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______.
 8. Seed from a progeny sesame plant having as a parent a sesame plant produced by the seed having been deposited under ATCC Accession No. ______.
 9. Tissue culture of seed having been deposited under ATCC Accession No. ______.
 10. Tissue culture of sesame plant S25 or its parts, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______. 11 A sesame plant regenerated from a tissue culture of a seed having been deposited under ATCC Accession No. ______, wherein said regenerated sesame plant has all the physiological and morphological characteristics of a sesame plant produced by said seed.
 12. A sesame plant regenerated from a tissue culture of sesame plant S25, a sample of the seed of sesame plant S25 having been deposited under ATCC Accession No. ______, wherein said regenerated sesame plant has all the physiological and morphological characteristics of said sesame plant S25.
 13. A method of producing sesame seed, comprising crossing a first parent sesame plant with a second parent sesame plant, wherein said first or second parent sesame plant was produced by seed having been deposited under ATCC Accession No. ______. 